LONG-TERM SUSPENSION-CULTURE OF TRITICUM-TIMOPHEEVII CELLS - 2 - KINETICS OF CELL-POPULATION

The size of the proliferative pool and the parameters of mitotic cycle (MC) were evaluated by the autoradiographic method in a rapidly growi ng long-term suspension culture of Triticum timopheevii (Zhuk.) Zhuk. cells. The proliferative pool reached 95%, MC was 17.5-h-long, and the mitosis itself lasted for 1 h. These temporal characteristics approac hed values typical of the apical meristematic cells in T. timopheevii root tips. However, other MC phases changed significantly: the G(1) pe riod was reduced almost completely and the S period was shortened twof old (to 2.5 h), whereas the G(2) period expanded twofold (to 14.03 h). The correlation between the kinetics of cell proliferation and the gr owth pattern in a long-subcultivated suspension culture of T. timophee vii is discussed.