AN IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY FOR SPIRONOLACTONE ANALYSIS

This study prepared an extemporaneously formulated liquid suspension d osage form (5 mg/ml) from commercially available 25 mg tablets. Stabil ity-indicating HPLC assay procedures were established and utilized to analyze the concentration of the drug. The method proved to be a simpl e model since it does not contain a buffer system. The mobile phase us ed was the same as that suggested by the manufacturer for the storage of the column. Therefore, the solvent system saves analytical processi ng time, since it does not require a change in the mobile phase before or after the analysis. The analytical method has been shown to be sta bility-indicating. The results have shown that there is no interferenc e from any of the degradation products obtained from stressing spirono lactone by heat and extremes in pH or with the internal standard, hydr ocortisone 21-acetate.