ESTABLISHMENT OF COMPLEMENT-RESISTANT RETROVIRAL VECTOR BY HOMOLOGOUSRESTRICTION FACTOR-20 GENE

Homologous restriction factor 20 (HRF20, CD59) is one of the complemen t regulatory factors. In this study, the complement-resistant retrovir al vector, which possesses the HRF20 gene as a selection gene, was con structed and examined. The virus-producing cell, transduced with compl ement-resistant retroviral vector, was established after complement-de pendent selection. NIH3T3 and PK15 cells transduced with this virus-pr oducing cell were successfully selected by complement-dependent select ion, which showed significant expression of the HRF20 antigen. In addi tion, these cells, transduced with tissue plasminogen activator (tPA) cDNA using complement-resistant retroviral vector, expressed tPA antig en after complement-dependent selection. These findings suggest that c omplement-resistant retroviral vector can be used for the double trans duction of HRF20, as well as other genes.