IMMOBILIZATION OF UREASE FROM PIGEONPEA (CAJANUS-CAJAN L.) IN POLYACRYLAMIDE GELS AND CALCIUM ALGINATE BEADS

Urease from pigeonpea was entrapped in polyacrylamide gel with 50 % im mobilization at 10 % total monomer (containing 5 % cross-linker) with high mechanical stability of the gel, Approximately 0.61 mg of protein could be loaded per 5 ml of gel, The immobilized enzyme had a t(1/2) of approx, 200 days when stored in 0.1 M Tris/acetate buffer, pH 6.5, at 4 degrees C, The gel strips were used 4 - 5 times for urea assay ov er a period of 6 h with less than 2 % loss of activity, Approximately 50 % immobilization of urease in calcium alginate was observed at 3 % alginate with 0.12 mg protein/ml alginate, The resultant enzyme beads showed a t(1/2) of approx, 75 days when stored in 0.1 M Tris/acetate b uffer, pH 6.5, at 4 degrees C, The beads were used 4 - 5 times for ure a assay over a period of 6 h with about 40 % loss of activity, In both cases, the enzyme activity was directly proportional to the amount of immobilized enzyme, There was practically no leaching of the entrappe d enzyme over a period of 48 h from either of the polymers, Both the i mmobilized enzyme preparations were used to analyse the blood urea of some clinical samples from the University hospital, The results obtain ed compared favourably with those obtained by the usual method employe d in the clinical pathology laboratory.