MICROSATELLITE DIMERIC, TRIMERIC, AND TETRAMERIC MOTIFS ON HUMAN-CHROMOSOME-3 - STUDY OF DISTRIBUTION AND DESIGN OF MARKERS

Nine microsatellite motifs were studied in DNA inserts of 150 NotI lin king and jumping clones of human chromosome 3, and also in the contig of 22 overlapping cosmids related to the region of homozygous deletion s in small-cell lung carcinoma (locus 3p21.3, 570 kbp). Compared with the human genome in total, the NotI clones had three times less CA rep eats and more GAG, GGA, AAT, CAC, AAAG, AATG, and GATG motifs, More th an 11 kbp of subcloned DNA fragments from clones NL1-212, NLM-216, NL- 026, J32-135H and cosmids 6, 7, and 16 were sequenced. Allele polymorp hism was studied in 10 microsatellite loci identified on human chromos ome 3 and in 8 CAG/CTG(n), loci of the brain cDNA (data from GenBank). Polymorphic loci were rather rare near the NotI sites (one of seven) and in cDNA (one of eight). This phenomenon and the distribution patte rns of some microsatellites suggest that the NotI clones of human chro mosome 3 and the cosmids related to the 3p21.3 region are rich in gene s. Two polymorphic and nine STS markers were generated for the human c hromosome 3; three of them were located in regions frequently affected in various solid tumors and four were related to genes. Polymorphic m arkers were related to the murine neuropeptide gene encoding receptor Y-YI gene (93.3% homology, 62 bp) and to the gene EST04896 expressed i n brain.