SURFACE-PLASMON RESONANCE MULTISENSING

We have demonstrated the feasibility of surface plasmon resonance (SPR ) multisensing by monitoring four separate immunoreactions simultaneou sly in real time using a multichannel SPR instrument. A plasmon carryi ng gold layer, onto which a four-channel now cell was pressed, was ima ged at a fixed angle of incidence. First, the four-channels were coate d with antibodies and then the flow cell, was turned by 90 degrees suc h that the flow channels overlapped the areas coated in the first step . In that geometry, antigens were applied to the different antibodies on the surface. Thus, all antibody-antigen combinations can be measure d in a two-dimensional away of sensor surfaces in real time. Our resul ts do correlate with expected immunologic specificity. The emphasis wi ll be on presenting this method to obtain data on immunosystems and no t as much on the assessment of biological activity.