B. Godlewskazylkiewicz et al., THE STUDY OF MAGNESIUM SPECIATION IN SERUM BY LIQUID-CHROMATOGRAPHY AND GRAPHITE-FURNACE ATOMIC-ABSORPTION TECHNIQUES, Analytica chimica acta, 358(2), 1998, pp. 185-193
The magnesium-protein complexes in human serum were separated and iden
tified using a liquid chromatographic anion-exchange column with diode
array detector for proteins and graphite furnace atomic absorption sp
ectrometry for magnesium. The conditions of separation were optimized
taking into account the best magnesium determination in the pyrolytica
lly coated graphite furnace. The serum sample was 2-fold diluted with
a Tris buffer and for gradient elution 0.5 mol l(-1) sodium acetate wa
s used. It was found that magnesium is mainly associated with albumin
and globulin fractions, but not with transferrin. Such separation was
possible only with fresh serum samples. (C) 1998 Elsevier Science B.V.