THE STUDY OF MAGNESIUM SPECIATION IN SERUM BY LIQUID-CHROMATOGRAPHY AND GRAPHITE-FURNACE ATOMIC-ABSORPTION TECHNIQUES

Citation
B. Godlewskazylkiewicz et al., THE STUDY OF MAGNESIUM SPECIATION IN SERUM BY LIQUID-CHROMATOGRAPHY AND GRAPHITE-FURNACE ATOMIC-ABSORPTION TECHNIQUES, Analytica chimica acta, 358(2), 1998, pp. 185-193
Citations number
17
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
00032670
Volume
358
Issue
2
Year of publication
1998
Pages
185 - 193
Database
ISI
SICI code
0003-2670(1998)358:2<185:TSOMSI>2.0.ZU;2-H
Abstract
The magnesium-protein complexes in human serum were separated and iden tified using a liquid chromatographic anion-exchange column with diode array detector for proteins and graphite furnace atomic absorption sp ectrometry for magnesium. The conditions of separation were optimized taking into account the best magnesium determination in the pyrolytica lly coated graphite furnace. The serum sample was 2-fold diluted with a Tris buffer and for gradient elution 0.5 mol l(-1) sodium acetate wa s used. It was found that magnesium is mainly associated with albumin and globulin fractions, but not with transferrin. Such separation was possible only with fresh serum samples. (C) 1998 Elsevier Science B.V.