MINING THE NATIONAL-CANCER-INSTITUTE ANTICANCER DRUG DISCOVERY DATABASE - CLUSTER-ANALYSIS OF ELLIPTICINE ANALOGS WITH P53-INVERSE AND CENTRAL NERVOUS SYSTEM-SELECTIVE PATTERNS OF ACTIVITY
Lm. Shi et al., MINING THE NATIONAL-CANCER-INSTITUTE ANTICANCER DRUG DISCOVERY DATABASE - CLUSTER-ANALYSIS OF ELLIPTICINE ANALOGS WITH P53-INVERSE AND CENTRAL NERVOUS SYSTEM-SELECTIVE PATTERNS OF ACTIVITY, Molecular pharmacology, 53(2), 1998, pp. 241-251
The United States National Cancer Institute conducts an anticancer dru
g discovery program in which similar to 10,000 compounds are screened
every year in vitro against a panel of 60 human cancer cell lines from
different organs. To date, similar to 62,000 compounds have been test
ed in the program, and a large amount of information on their activity
patterns has been accumulated. For the current study, anticancer acti
vity patterns of 112 ellipticine analogs were analyzed with the use of
a hierarchical clustering algorithm. A dramatic coherence between mol
ecular structures and their activity patterns could be seen from the c
luster tree: the first subgroup (compounds 1-66) consisted principally
of normal ellipticines, whereas the second subgroup (compounds 67-112
) consisted principally of N-2-alkyl-substituted ellipticiniums. Almos
t all apparent discrepancies in this clustering were explainable on th
e basis of chemical transformation to active forms under cell culture
conditions. Correlations of activity with p53 status and selective act
ivity against cells of central nervous system origin made this data se
t of special interest to us. The ellipticiniums, but not the elliptici
nes, were more potent on average against p53 mutant cells than against
p53 wild-type ones (i.e., they seemed to be ''p53-inverse'') in this
short term assay. This study strongly supports the hypothesis that ''f
ingerprint'' patterns of activity in the National Cancer Institute in
vitro cell screening program encode incisive information on the mechan
isms of action and other biological behaviors of tested compounds. Ins
ights gained by mining the activity patterns could contribute to our u
nderstanding of anticancer drugs and the molecular pharmacology of can
cer.