HIGH-TEMPERATURE CONJUGATION OF PROTEINS WITH CARBOHYDRATES

Authors
BORATYNSKI J ROY R
Citation
J. Boratynski et R. Roy, HIGH-TEMPERATURE CONJUGATION OF PROTEINS WITH CARBOHYDRATES, Glycoconjugate journal, 15(2), 1998, pp. 131-138
Citations number
13
Categorie Soggetti
Biology
Journal title
Glycoconjugate journal → ACNP
ISSN journal
02820080
Volume
15
Issue
2
Year of publication
1998
Pages
131 - 138
Database
ISI
SICI code
0282-0080(1998)15:2<131:HCOPWC>2.0.ZU;2-F
Abstract
A new procedure was used to conjugate lactose and dextran with BSA wit hout using coupling or activating reagents. The method is simple, rapi d and cheap. Reducing sugars covalently bind to proteins when lyophili zed together and briefly heated to a high temperature.