COMBINED PREPARATIVE ENZYMATIC-SYNTHESIS OF DTDP-6-DEOXY-4-KETO-D-GLUCOSE FROM DTDP AND SUCROSE

dTDP-6-deoxy-4-keto-D-glucose (1), the common intermediate in the bios yntheses of the manifold deoxysugars, was synthesized on a gram-scale by the combination of sucrose synthase and dTDP-D-glucose 4,6-dehydrat ase in a fed batch, starting the reaction with dTDP. This process allo wed a dTDP conversion with a 100% rate. An easy and efficient three-st ep purification with anion-exchange chromatography and gel filtration gave 1.1 g of 1 in an overall yield of 73%. This work realizes a first step for an economic access to activated deoxysugars.