H. Kaneko et al., EXPRESSION OF PAX5 GENE IN HUMAN HEMATOPOIETIC-CELLS AND TISSUES - COMPARISON WITH IMMUNODEFICIENT DONORS, Clinical and experimental immunology, 111(2), 1998, pp. 339-344
In mice, Pax5 gene is indispensable for B cell development. Pax5-defic
ient mice fail to produce mature B cells owing to complete arrest of B
cell development at a precursor stage. However, the lineage and stage
of human Pax5 gene expression have remained elusive. In this investig
ation expression of the human Pax5 gene was studied. Pax5 gene express
ion was detected in B cell lines but not in myeloma cell lines. CD19 e
xpression was correlated with Pax5 gene expression. Adult spleen and b
one marrow and fetal spleen and Liver showed strong Pax5 gene expressi
on, as did the corresponding mouse tissues, as reported previously. In
common variable immunodeficiency (CVID) peripheral blood lymphocytes
(PBL) with a decreased number of B cells, no Pax5 gene expression was
detected. Some CVID PBL stimulated with IL-2, IL-IO and anti-CD40 mono
clonal antibody, expressed the Pax5 gene. Defect of Pax5 gene expressi
on in CVID may be caused by regulatory T cell disorder.