LOW CATALASE ACTIVITY IN RATS WITH URETERAL LIGATION - RELATION TO LIPID-PEROXIDATION

Progression of some renal diseases is characterized by generation of r eactive oxygen metabolites that are also involved in the pathophysiolo gy of obstructive nephropathy, Catalase activity and lipid peroxidatio n were investigated in rats with unilaterally (UUL) and bilaterally li gated ureters (BUL). Forty-eight hours after ligation, the animals wer e sacrificed, and enzyme activity as well as the malondialdehyde (MDA) concentration were measured in the plasma, kidneys and livers, The ac tivity of catalase was significantly reduced in the plasma of the BUL rats and in the kidneys of both investigated groups, In the liver, cat alase activity was decreased only in the BUL group, The MDA concentrat ion in the plasma and kidneys of the BUL rats was significantly increa sed while in the liver it remained unchanged, These results suggest th at lipid peroxidation in the induced uremic state could be responsible for catalase inactivation.