IMPROVED RESOLUTION OF MONOVINYL AND DIVINYL CHLOROPHYLL-A AND CHLOROPHYLL-B AND ZEAXANTHIN AND LUTEIN IN PHYTOPLANKTON EXTRACTS USING REVERSE-PHASE C-8 HPLC

Authors
BARLOW RG CUMMINGS DG GIBB SW
Citation
Rg. Barlow et al., IMPROVED RESOLUTION OF MONOVINYL AND DIVINYL CHLOROPHYLL-A AND CHLOROPHYLL-B AND ZEAXANTHIN AND LUTEIN IN PHYTOPLANKTON EXTRACTS USING REVERSE-PHASE C-8 HPLC, Marine ecology. Progress series, 161, 1997, pp. 303-307
Citations number
19
Journal title
Marine ecology. Progress series → ACNP
ISSN journal
01718630
Volume
161
Year of publication
1997
Pages
303 - 307
Database
ISI
SICI code
0171-8630(1997)161:<303:IROMAD>2.0.ZU;2-U
Abstract
A reverse phase HPLC (high performance liquid chromatography) techniqu e using a binary solvent system with a linear gradient on a Hypersil(R ) MOS2 C-8 column is described. As well as the resolution of key chemo taxonomic chlorophyll and carotenoid pigments, baseline separation of mono-and divinyl chlorophyll a and of lutein and zeaxanthin, and parti al separation of mono-and divinyl chlorophyll b are achieved in a tota l analysis time of less than 30 min. The method provides an optimal ba lance between analyte resolution and sample throughput and is hence we ll suited to the analysis of oceanographic samples.