PRACTICAL APPROACH FOR DETECTION AND IDENTIFICATION OF OXA-10-DERIVEDCEFTAZIDIME-HYDROLYZING EXTENDED-SPECTRUM BETA-LACTAMASES

Authors
VAHABOGLU H OZTURK R AKBAL H SARIBAS S TANSEL O COSKUNKAN F
Citation
H. Vahaboglu et al., PRACTICAL APPROACH FOR DETECTION AND IDENTIFICATION OF OXA-10-DERIVEDCEFTAZIDIME-HYDROLYZING EXTENDED-SPECTRUM BETA-LACTAMASES, Journal of clinical microbiology, 36(3), 1998, pp. 827-829
Citations number
8
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
36
Issue
3
Year of publication
1998
Pages
827 - 829
Database
ISI
SICI code
0095-1137(1998)36:3<827:PAFDAI>2.0.ZU;2-7
Abstract
A practical approach to detect and identify ceftazidime-hydrolyzing ex tended-spectrum mutants of OXA-10 beta-lactamase is presented. Large n umbers of bacteria were screened by colony hybridization, a 720-bp par t of bla(OXA) was amplified by PCR from the hybridization-positive iso lates, and the products were digested by PvuII and HaeIII.