H. Vahaboglu et al., PRACTICAL APPROACH FOR DETECTION AND IDENTIFICATION OF OXA-10-DERIVEDCEFTAZIDIME-HYDROLYZING EXTENDED-SPECTRUM BETA-LACTAMASES, Journal of clinical microbiology, 36(3), 1998, pp. 827-829
A practical approach to detect and identify ceftazidime-hydrolyzing ex
tended-spectrum mutants of OXA-10 beta-lactamase is presented. Large n
umbers of bacteria were screened by colony hybridization, a 720-bp par
t of bla(OXA) was amplified by PCR from the hybridization-positive iso
lates, and the products were digested by PvuII and HaeIII.