GRAFTS OF OVARIES IN MALES AND FEMALES OF PLEURODELES WALTL (URODELE AMPHIBIA) - EVIDENCE OF A LONG-TERM TOLERANCE OF ALLOGRAFTS - APPLICATION FOLLOWING A SPACE BIOLOGY EXPERIMENT
C. Dournon et al., GRAFTS OF OVARIES IN MALES AND FEMALES OF PLEURODELES WALTL (URODELE AMPHIBIA) - EVIDENCE OF A LONG-TERM TOLERANCE OF ALLOGRAFTS - APPLICATION FOLLOWING A SPACE BIOLOGY EXPERIMENT, Reproduction, nutrition, development, 37(6), 1997, pp. 637-649
Ovary grafts were investigated in the salamander Pleurodeles using juv
eniles and adults as donors and hosts. Ovaries were provided by standa
rd cr histo-compatible strains and by standard females which had been
submitted to a space flight. Laparotomy of the hosts was used to contr
ol viability of grafts. Entire juvenile ovaries transplanted into cast
rated juvenile females or males were tolerated and developed. Ovarian
parts of adult females, which contained a majority of oogonies, could
also be tolerated by juvenile animals. In addition, ovarian parts supp
orted a better recovery and differentiation than parts that mainly inc
luded mature oocytes. About 24 months after the ovary grafts, some hos
ts (genetical females or males and standard or spatialized females) cr
ossed with standard males provided progenies originating from oocytes
of the grafted ovaries. The protocols applied offer a new range of pot
entialities, adapted to various experimental purposes such as life sci
ence research in space or sex differentiation studies.