HYDROGEN-EXCHANGE STUDIES OF PROTEIN-STRUCTURE

Hydrogen exchange techniques, with their residue-level specificity, ex quisite sensitivity, and adaptability to many solution conditions, are becoming essential to the study of protein stability, folding and dyn amics. Recent studies have elucidated the structures of intermediates formed transiently during protein folding and rare partially folded en sembles present at equilibrium. Analysis of hydrogen exchange mechanis ms has revealed protein stability and kinetics at the level of individ ual residues. (C) Current Biology Ltd ISSN 0958-1669.