PHYLOGENETIC ANALYSIS AND IDENTIFICATION OF SHIGELLA SPP. BY MOLECULAR PROBES

Citation
Rf. Wang et al., PHYLOGENETIC ANALYSIS AND IDENTIFICATION OF SHIGELLA SPP. BY MOLECULAR PROBES, Molecular and cellular probes, 11(6), 1997, pp. 427-432
Citations number
22
Categorie Soggetti
Biology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
ISSN journal
08908508
Volume
11
Issue
6
Year of publication
1997
Pages
427 - 432
Database
ISI
SICI code
0890-8508(1997)11:6<427:PAAIOS>2.0.ZU;2-G
Abstract
Oligonucleotide probes were used for identification of Shigella and an alysis of the relationship between Shigella spp. and Escherichia coil. Probe-based PCRs shown cross-reactions from Shigella to E. coil. Prob e-based 16S rRNA sequencing and phylogenetic analysis showed the four species of Shigella: Sh. dysenteriae, Sh. boydii, Sh. sonnei, and Sh. flexneri, formed a cluster with E. coil. Shigella flexneri and Sh. son nei are even more similar to E. coli than to the other two Shigella sp ecies. These results confirmed an earlier recommendation that the four species of Shigella and E. coil should be classified as five sub-grou ps within a single species. (C) 1997 Academic Press Limited.