GENERATION OF SACCHAROMYCES-CEREVISIAE DELETANTS AND BASIC PHENOTYPICANALYSIS OF 8 NOVEL GENES FROM THE LEFT ARM OF CHROMOSOME-XIV

The disruption of eight novel genes was realized in two genetic backgr ounds. Among these open reading frames, NO333, NO348 and NO364 present ed homologies with other proteins of yeast or other organisms, whereas NO320, NO325, NO339, NO384 and NO388 showed no similarity with any pr otein. Tetrad analysis of heterozygous deletant strains revealed that NO348, NO364 and NO388 are essential genes for vegetative growth, wher eas NO320, NO325, NO333, NO339 and N0384 are non-essential. Basic phen otypic analyses of the non-lethal deletant strains as suggested in the six-pack B0 programme did not reveal any significant differences betw een parental and mutant strains. (C) 1998 John Wiley & Sons, Ltd.