THE SPL1 TRANSFER-RNA SPLICING GENE OF CANDIDA-MALTOSA AND CANDIDA-ALBICANS

The tRNA splicing gene SPL1-1 has been cloned and sequenced in Sacchar omyces cerevisiae (Kolman and Sell, 1993). Sequence adjacent to the LE U2 gene in Candida maltosa showed some homology to the SPL1-1 gene of S. cerevisiae. This work describes the sequencing of the SPL1 tRNA spl icing genes from C. maltosa and C. albicans and the analysis of these genes. Comparison of these sequences and the relationship observed bet ween the LEU2 and SPL1 genes in these yeasts suggests that there may b e some synteny amongst various species of yeasts. The coding region of the C. maltosa SPL1 region described in this work differs from previo usly described partial sequences in that it is a complete uninterrupte d open reading frame. Two strains of C. maltosa were each shown to con tain different alleles, one uninterrupted open reading frame and one d isrupted open reading frame. The sequences have been deposited in the GenBank/EMBL data libraries under Accession Numbers X72940, AF000115, AF000116, AF000117, AF000118, AF000119 and AF000120. (C) 1998 John Wil ey & Sons, Ltd.