ELECTROGENERATED CHEMILUMINESCENCE FOR DETERMINATION OF INDOLE AND TRYPTOPHAN

Indole and tryptophan produce electrogenerated chemiluminescence (ECL) at a Pt electrode under triangular pulse voltage when using KCl as th e supporting electrolyte; the intensity was enhanced in the presence o f ca. 10(-2) mol l(-1) H2O2. The effects of electrochemical parameters , solution and interference conditions are investigated in detail. Und er the optimum conditions, the detection limit (signal:noise=3) for in dole and tryptophan was 1 x 10(-7) mol l(-1), with the linear calibrat ion range extending up to 8 x 10(-5) mol l(-1).