MYCOTOXINS IN INGREDIENTS OF ANIMAL FEEDING STUFFS - III - DETERMINATION OF MYCOTOXINS IN RICE BRAN

Methods used previously for the determination of aflatoxins B-1, B-2, G(1) and G(2), ochratoxins A and B, cyclopiazonic acid, zearalenone, s terigmatocystin, and moniliformin in maize were applied successfully t o rice bran. However, recovery of deoxynivalenol and other trichothece ne mycotoxins spiked into samples was lower than expected and Mo citri nin could be recovered. Forty samples of rice bran used in the animal feed industry were examined for the presence of 20 mycotoxins. The lev el of contamination of rice bran by mycotoxins was low. Aflatoxin B-1 was present in 29 (72.5%) samples, usually together with related aflat oxins, up to a fetal of 28 mu g/kg after allowing for recovery losses. Ochratoxin A at a level of 12 and 3 mu g/kg was confirmed in two samp les, while most samples also appeared to contain small concentrations of ochratoxin A. A chromatographic peak coresponding to cyclopiazonic acid occurred in several samples and one sample appeared to contain a low level of moniliformin. Positive confirmation of these last two myc otoxins was difficult and these findings must be regarded with caution . No other toxins were detected.