LIPOPOLYSACCHARIDE INDUCES APOPTOSIS IN A BOVINE ENDOTHELIAL-CELL LINE VIA A SOLUBLE CD14 DEPENDENT PATHWAY

Authors
FREY EA FINLAY BB
Citation
Ea. Frey et Bb. Finlay, LIPOPOLYSACCHARIDE INDUCES APOPTOSIS IN A BOVINE ENDOTHELIAL-CELL LINE VIA A SOLUBLE CD14 DEPENDENT PATHWAY, Microbial pathogenesis, 24(2), 1998, pp. 101-109
Citations number
37
Categorie Soggetti
Immunology,Microbiology
Journal title
Microbial pathogenesis → ACNP
ISSN journal
08824010
Volume
24
Issue
2
Year of publication
1998
Pages
101 - 109
Database
ISI
SICI code
0882-4010(1998)24:2<101:LIAIAB>2.0.ZU;2-E
Abstract
We investigated the mode of bovine endothelial cell death induced by l ipopolysaccharide (LPS). Inter-nucleosomal DNA fragmentation was obser ved indicating apoptotic cell death. Apoptosis was confirmed by examin ing cell morphology using confocal laser scanning and transmission ele ctron microscopy, which revealed characteristic nuclear condensation i n LPS-treated endothelial cells. Apoptosis was blocked by a monoclonal antibody against CD14, suggesting that LPS triggers apoptosis via a s oluble CD14 (sCD14) dependent mechanism. (C) 1998 Academic Press Limit ed.