IDENTIFICATION OF PORCINE PNEUMOCYSTIS-CARINII AS A GENETICALLY DISTINCT ORGANISM BY DNA AMPLIFICATION

Authors
WAKEFIELD AE KEELY SP STRINGER JR CHRISTENSEN CBV AHRENS P PETERS SE BILLEHANSEN V HENRIKSEN SA JORSAL SE SETTNES OP
Citation
Ae. Wakefield et al., IDENTIFICATION OF PORCINE PNEUMOCYSTIS-CARINII AS A GENETICALLY DISTINCT ORGANISM BY DNA AMPLIFICATION, APMIS. Acta pathologica, microbiologica et immunologica Scandinavica, 105(4), 1997, pp. 317-321
Citations number
40
Categorie Soggetti
Pathology,Microbiology,Immunology
Journal title
APMIS. Acta pathologica, microbiologica et immunologica Scandinavica → ACNP
ISSN journal
09034641
Volume
105
Issue
4
Year of publication
1997
Pages
317 - 321
Database
ISI
SICI code
0903-4641(1997)105:4<317:IOPPAA>2.0.ZU;2-N
Abstract
DNA was amplified from lung samples from three piglets infected with P neumocystis carinii, using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated la ck of sequence variation among these pig-derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically d istinct from P. carinii isolated from other mammalian host species.