LOCALIZATION OF AN INSULIN-LIKE-GROWTH-FACTOR (IGF) BINDING-SITE OF BOVINE IGF BINDING PROTEIN-2 USING DISULFIDE MAPPING AND DELETION MUTATION ANALYSIS OF THE C-TERMINAL DOMAIN

We have investigated which region(s) of bovine insulin-like growth fac tor binding protein-2 (bIGFBP-2) interact with insulin-like growth fac tors (IGFs) using C-terminally truncated forms of bIGFBP-2. Initially to aid in mutant design, we defined the disulfide bonding pattern of b IGFBP-2 C-terminal region using enzymatic digestion, The pattern is Cy s(186)-Cys(220), CyS231-CyS242, and Cys(244)-Cys(265). I, addition, cy anogen bromide cleavage of bIGFBP-2 revealed that the N-and C-terminal cysteine-rich domains were not linked by disulfide bonds, Taking the disulfide bonding pattern into consideration, C-terminal truncation mu tants were designed and expressed in COS-1 mammalian cells, Following IGF binding assays, a region between residues 222 and 236 was identifi ed as important in IGF binding, Specifically, mutants truncated by 14, 36, and 48 residues from the C terminus bound IGFs to the same extent as wild type (WT) bIGFBP-2. Removal of 63 residues resulted in a grea tly reduced (up to 80-fold) ability to bind IGF compared with WT bIGFB P-2. Interestingly this mutant lacked the IGF-II binding preference of WT bIGFBP-2. Residues 236-270 also appeared to play a role in determi ning IGF binding specificity as their removal resulted in mutants with higher IGF-II binding affinity.