A. Moro et al., EFFECT OF CYCLINS AND CDKS ON THE CYCLIN B1 PROMOTER ACTIVATION, Biochemistry and molecular biology international, 41(5), 1997, pp. 919-924
To study the effect of several cyclins on cyclin B1 promoter activatio
n, we cotransfected cyclin A, cyclin E and cyclin D1 expressing plasmi
ds with a cyclin B1 promoter construction driving a luciferase reporte
r gene into NIH 3T3 cells. All three cyclins produced activation of th
e reporter gene, however, co-transfection of cyclin A with a Cdk2 domi
nant negative mutant blocks activation by cyclin A. Our results sugges
t that cyclin B1 activation depends on Cdk2 kinase activity associated
to cyclin A. To our knowledge this is the first report showing positi
ve effect of cyclin A, cyclin E and cyclin D1 on cyclin B1 activation
and blocking of this activation by a Cdk2 dominant negative mutant.