EFFECT OF CYCLINS AND CDKS ON THE CYCLIN B1 PROMOTER ACTIVATION

Citation
A. Moro et al., EFFECT OF CYCLINS AND CDKS ON THE CYCLIN B1 PROMOTER ACTIVATION, Biochemistry and molecular biology international, 41(5), 1997, pp. 919-924
Citations number
23
Categorie Soggetti
Biology
ISSN journal
10399712
Volume
41
Issue
5
Year of publication
1997
Pages
919 - 924
Database
ISI
SICI code
1039-9712(1997)41:5<919:EOCACO>2.0.ZU;2-J
Abstract
To study the effect of several cyclins on cyclin B1 promoter activatio n, we cotransfected cyclin A, cyclin E and cyclin D1 expressing plasmi ds with a cyclin B1 promoter construction driving a luciferase reporte r gene into NIH 3T3 cells. All three cyclins produced activation of th e reporter gene, however, co-transfection of cyclin A with a Cdk2 domi nant negative mutant blocks activation by cyclin A. Our results sugges t that cyclin B1 activation depends on Cdk2 kinase activity associated to cyclin A. To our knowledge this is the first report showing positi ve effect of cyclin A, cyclin E and cyclin D1 on cyclin B1 activation and blocking of this activation by a Cdk2 dominant negative mutant.