PURIFICATION OF PROTEINS BY MEMBRANE CHROMATOGRAPHY

Adsorption chromatography is increasingly used for protein purificatio n and medical applications. Synthetic membranes have advantages as sup port matrices in comparison to conventional bead supports because they are not compressible and they eliminate diffusion Limitations. As a r esult, higher throughput and faster processing times are possible in m embrane systems. In this paper, we review the various applications of membrane chromatography by focusing on affinity, ion exchange, hydroph obic interaction, reversed-phase and multistage chromatography. The pr ospects of further development of membrane chromatography are discusse d. (C) 1998 SCI.