W. Khunkitti et al., ACANTHAMOEBA-CASTELLANII - GROWTH, ENCYSTMENT, EXCYSTMENT AND BIOCIDESUSCEPTIBILITY, The Journal of infection, 36(1), 1998, pp. 43-48
Stages in the encystment and excystment processes of Acanthamoeba cast
ellanii have been studied, The kinetics of encystment involved measure
ments of the three phases (pre-encystment, cyst initiation and cyst wa
ll synthesis), Excystment, starting from a mature cyst, involved pre-e
mergence, penetration outwards of the cyst wall and free trophozoite,
The sensitivity to biocides of trophozoites in the exponential growth
phase, pre-encystment trophozoites, mature cysts and pre-excystment cy
sts has been investigated, Some differences in relative sensitivity to
a bisbiguanide (chlorhexidine) and a polymeric biguanide (polyhexamet
hylene biguanide) were observed, but mature cysts were always the most
resistant cellular form.