S. Chand et P. Basu, EMBRYOGENESIS AND PLANT-REGENERATION FROM CALLUS-CULTURES DERIVED FROM UNPOLLINATED OVARIES OF HYOSCYAMUS-MUTICUS L, Plant cell reports, 17(4), 1998, pp. 302-305
Unpollinated ovaries of Hyoscyamus muticus L. (commonly known as Egypt
ian henbane) were cultured on Murashige and Skoog and Bourgin and Nits
ch media supplemented with various growth hormones to study the organo
genesis, embryogenesis and regeneration of plantlets, Embryogenesis wa
s reported for callus grown on both media containing 0.05 mg/l alpha-n
aphthaleneacetic acid and 0.5 mg/l 6-benzylaminopurine, Differentiatio
n of roots and shoots from the calli also occurred in these media, Alb
inism or chlorophyll deficiency and variation in ploidy level were obs
erved among the ovary-derived plantlets.