PARATHYROID-HORMONE ACTIVATION OF THE 25-HYDROXYVITAMIN D-3-1-ALPHA-HYDROXYLASE GENE PROMOTER

Citation
Hl. Brenza et al., PARATHYROID-HORMONE ACTIVATION OF THE 25-HYDROXYVITAMIN D-3-1-ALPHA-HYDROXYLASE GENE PROMOTER, Proceedings of the National Academy of Sciences of the United Statesof America, 95(4), 1998, pp. 1387-1391
Citations number
29
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
95
Issue
4
Year of publication
1998
Pages
1387 - 1391
Database
ISI
SICI code
0027-8424(1998)95:4<1387:PAOT2D>2.0.ZU;2-M
Abstract
The DNA flanking the 5' sequence of the mouse 1 alpha-hydroxylase gene has been cloned and sequenced. A TATA box has been located at -30 bp and aCCAAT box has been located at -79 bp. The gene's promoter activit y has br!en demonstrated by using a luciferase reporter gene construct transfected into a modified pig kidney cell line, AOK-B50. Parathyroi d hormone stimulates this promoter-directed synthesis of luciferase by 17-fold, whereas forskolin stimulates it by 3-fold. The action of par athyroid hormone is concentration-dependent. 1,25-Dihydroxyvitamin D-3 does not suppress basal promoter activity and marginally suppresses p arathyroid hormone-driven luciferase reporter activity. The promoter h as three potential cAMP-responsive element sites, and two perfect and one imperfect AP-1 sites, while no DR-3 was detected. These results in dicate that parathyroid hormone stimulates 25-hydroxyvitamin D-3-1 alp ha-hydroxylase by acting on the promoter of the 1 alpha-hydroxylase ge ne.