MOLECULAR PICTURE OF FOLDING OF A SMALL ALPHA BETA PROTEIN/

We characterize, at the atomic level, the mechanism and thermodynamics of folding of a small alpha/beta protein, The thermodynamically signi ficant states of segment B1 of streptococcal protein G (GB1) are probe d by using the statistical mechanical methods of importance sampling a nd molecular dynamics, From a thermodynamic standpoint, folding commen ces with overall collapse, accompanied hy formation of similar to 35% of the native structure. Specific contacts form at the loci experiment ally inferred to be structured early in folding kinetics studies, Our study reveals that these initially structured regions are not spatiall y adjacent, As folding progresses, fluid-like nonlocal native contacts form, with many contacts forming and breaking as the structure search es for the native conformation, Although the alpha-helix forms early, the beta-sheet forms concomitantly with the overall topology. Water is present in the protein core up to a late stage of folding, lubricatin g conformational transitions during the search process. Once 80% of th e native contacts have formed, water is squeezed from the protein inte rior and the structure descends into the native manifold, Examination of the onset of side-chain mobility within our model indicates side-ch ain motion is most closely linked to the overall volume of the protein and no sharp order-disorder transition appears to occur. Exploration of models for hydrogen deuterium exchange show qualitative agreement w ith equilibrium measurement of hydrogen/deuterium protection factors.