HIV-1 SIMIAN IMMUNODEFICIENCY VIRUS-INFECTION OF HUMAN AND NONHUMAN PRIMATE LYMPHOCYTES RESULTS IN THE MIGRATION OF CD2(-CELLS INTO THE INTESTINE OF ENGRAFTED SCID MICE() T)
Hh. Donze et al., HIV-1 SIMIAN IMMUNODEFICIENCY VIRUS-INFECTION OF HUMAN AND NONHUMAN PRIMATE LYMPHOCYTES RESULTS IN THE MIGRATION OF CD2(-CELLS INTO THE INTESTINE OF ENGRAFTED SCID MICE() T), The Journal of immunology, 160(5), 1998, pp. 2506-2513
Increased lymphocytic infiltration of intestinal tissues has been obse
rved in patients infected with HIV-1 and in SIV-infected rhesus macaqu
es. To determine whether HIV-1 and SIV infections influence the homing
of human and nonhuman primate PBMC to intestinal tissues, we engrafte
d SCID mice with human or nonhuman primate PBMC and infected them with
either cell-free or cell-associated HIV-1 or SIV, In mice that receiv
ed both PBMC and virus, human or nonhuman primate CD2(+) T cells were
found in intestinal tissues, primarily in the intraepithelial lymphocy
te compartment and lamina propria, Immunomagnetic sorting revealed tha
t these cells were derived from the CD4(+) population, Using gag-speci
fic primers, PCR analysis of these tissues detected the presence of HI
V-1 proviral DNA, However, in SCID mice that were engrafted with eithe
r human or nonhuman primate PBMC and no HIV-1 or SIV, CD2(+) T cells w
ere not detected in intestinal tissues, These results indicate that HI
V-1 and SIV can modulate the migratory properties of human and nonhuma
n primate T cells in the SCID mouse model.