Jw. Defijter et al., DECREASED CYTOKINE-INDUCED IGA SUBCLASS PRODUCTION BY CD40-LIGATED CIRCULATING B-CELLS IN PRIMARY IGA NEPHROPATHY, Nephrology, dialysis, transplantation, 13(2), 1998, pp. 285-292
Background. In IgA nephropathy (IgAN), the abnormalities in the IgA im
mune system are apparently restricted to the IgA1 subclass in the syst
emic compartment, as evidenced by the antigen-specific responses to re
call antigens. Since precursors of IgA producing B cells in human peri
pheral blood belong predominantly to the mucosal compartment, we took
the opportunity to assess the capacity of circulating B cells in perip
heral blood (PBMC) of 20 IgAN patients and matched controls to produce
IEA, IgA1, and IgA2. Methods. Supernatants from T cell- (immobilized
anti-CD3) and B cell-specific (CD40 ligation) activated cultures were
assessed for immunoglobulin isotypes by ELISA. In addition, we compare
d the sensitivity of T and B cells to various cytokines (IL-2, IL-10,
TGF-beta) in both culture systems. Results. In contrast to significant
ly higher plasma IgA1 levels (P<0.01), no significant differences in s
alivary IgA1 (P=0.73) and IgA2 (P=0.96) levels or ratios (P=0.91) were
found. In the absence of exogenous cytokines, none of the different c
ulture systems led to significant differences in IgA or IgA subclass s
ynthesis by PBMCs of patients and controls. However, in IgAN patients,
the addition of IL-2 did not enhance the production of the IgA subcla
sses as was found in controls. Furthermore IL-10 led to significantly
(P<0.05) lower IgA1 and IgA2 synthesis in patients than in controls. T
GF-beta induced suppression of all isotypes in patients and controls.
None of the different conditions resulted in a selectively enhanced pr
oduction of any one of the IgA subclasses. When both IL-10 and TGF-bet
a were added to the cultures, IgM was the predominant immunoglobulin s
ynthesized both in patients and controls with a significantly (P<0.05)
lower synthesis of IgM, IgG, IgA1, and IgA2 in patients. Conclusion.
These in vitro data suggest that PBMCs from patients contain more matu
re and further differentiated B cells. However, there was no selective
IgA or IgA1 dysregulation of circulating B cells in IgAN. These resul
ts do not confirm the widely believed paradigm that patients with IgAN
are primary hyperresponders.