Qz. Du et al., SEPARATION OF EPIGALLOCATECHIN GALLATE AND GALLOCATECHIN GALLATE USING MULTIPLE INSTRUMENTS CONNECTED IN SERIES, Journal of liquid chromatography & related technologies, 21(1-2), 1998, pp. 203-208
Citations number
2
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A synthetic mixture of epigallocatechin gallate and gallocatechin gall
ate was separated using multiple countercurrent chromatographic instru
ments by connecting the separation columns in series. Peak resolution
increased according to the formula Rs-n = n(1/2)R(s-1), where n is the
number of columns connected; Rs-n and Rs-1, the peak resolution obtai
ned from n columns and a single column, respectively. Various sample s
izes and concentrations were applied to four columns connected in seri
es. The results indicated that the sample loading capacity is increase
d 11 times that of the single instrument for the same peak resolution.