SEPARATION OF EPIGALLOCATECHIN GALLATE AND GALLOCATECHIN GALLATE USING MULTIPLE INSTRUMENTS CONNECTED IN SERIES

A synthetic mixture of epigallocatechin gallate and gallocatechin gall ate was separated using multiple countercurrent chromatographic instru ments by connecting the separation columns in series. Peak resolution increased according to the formula Rs-n = n(1/2)R(s-1), where n is the number of columns connected; Rs-n and Rs-1, the peak resolution obtai ned from n columns and a single column, respectively. Various sample s izes and concentrations were applied to four columns connected in seri es. The results indicated that the sample loading capacity is increase d 11 times that of the single instrument for the same peak resolution.