A RAPID AND SIMPLIFIED EXTRACTION METHOD OF ERYTHROMYCIN FROM FERMENTATION BROTH WITH BOND ELUT C18 CARTRIDGE FOR ANALYSIS BY HPLC

Authors
HEYDARIAN SM ISON AP MIRJALILI N
Citation
Sm. Heydarian et al., A RAPID AND SIMPLIFIED EXTRACTION METHOD OF ERYTHROMYCIN FROM FERMENTATION BROTH WITH BOND ELUT C18 CARTRIDGE FOR ANALYSIS BY HPLC, Biotechnology techniques, 12(2), 1998, pp. 155-158
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
2
Year of publication
1998
Pages
155 - 158
Database
ISI
SICI code
0951-208X(1998)12:2<155:ARASEM>2.0.ZU;2-K
Abstract
Solid phase extraction of erythromycin from fermentation broth is desc ribed in this paper. This method results in 85 percent recovery of ery thromycin A with a batch to batch variability of less than 3 percent. Each column can be used twice with 2-3% loss of recovery. The columns are regenerated immediately after each extraction. In comparison with liquid liquid extraction, solid phase extracts are cleaner, the method is less time consuming and exposure to hazardous solvents is reduced.