ELECTROPORATION OF NUCLEIC-ACIDS INTO PROKARYOTIC AND EUKARYOTIC CELLS BY SQUARE-WAVE PULSES

Authors
HANZE J FISCHER L KOENEN M WORGALL S RASCHER W
Citation
J. Hanze et al., ELECTROPORATION OF NUCLEIC-ACIDS INTO PROKARYOTIC AND EUKARYOTIC CELLS BY SQUARE-WAVE PULSES, Biotechnology techniques, 12(2), 1998, pp. 159-163
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
2
Year of publication
1998
Pages
159 - 163
Database
ISI
SICI code
0951-208X(1998)12:2<159:EONIPA>2.0.ZU;2-Q
Abstract
A novel electroporator is presented that generates adjustable square w ave pulses with a maximal pulse strength of 2500 V/100 A and a pulse l ength between 0.1 and 15 ms and that efficiently transfects prokaryoti c and eukaryotic cells. Various factors influencing DNA transfer into tissue culture cells, embryonic stem cells and E. coli K12 cells were optimized and the respective electrical pulse profiles monitored.