SENSITIVE AND REPRODUCIBLE METHOD FOR NEUTRAL MONOSACCHARIDES AND URONIC-ACID DETERMINATION IN SUGAR-BEET PULP ENZYMATIC TREATMENT MEDIA AND PRESS JUICES BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION

Authors
GAILING MF GUIBERT A COMBES D
Citation
Mf. Gailing et al., SENSITIVE AND REPRODUCIBLE METHOD FOR NEUTRAL MONOSACCHARIDES AND URONIC-ACID DETERMINATION IN SUGAR-BEET PULP ENZYMATIC TREATMENT MEDIA AND PRESS JUICES BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION, Biotechnology techniques, 12(2), 1998, pp. 165-169
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
2
Year of publication
1998
Pages
165 - 169
Database
ISI
SICI code
0951-208X(1998)12:2<165:SARMFN>2.0.ZU;2-A
Abstract
The resolution and the elution of arabinose, rhamnose, xylose, glucose , galactose, fructose, sucrose and galacturonic acid by an High Perfor mance Anion Exchange Chromatography method were studied. The best resu lts were obtained with a two step elution composed of a 25mM NaOH isoc ratic phase for 20 min followed by a 300mM sodium acetate gradient for 10 min. The reliability, repeatability and sensitivity of these analy tical conditions were set.