RAPID MAPPING OF MARKERS APPLYING VECTORETTE TECHNOLOGY TO YAC FRAGMENTATION ALLOWS EASY ASSEMBLY OF A HIGH-DENSITY STS BACTERIAL CLONE CONTIG SPANNING THE MARKERS D6S1260-D6S1918

We have generated a detailed physical map of the 6p21.3/p22.1 boundary , using a combination of yeast artificial chromosome (YAC) fragmentati on and high resolution sequence tagged site (STS) content mapping. YAC s from the CEPH, St. Louis, and ICRF libraries have been used to const ruct a 4.5-Mb contig spanning the markers D6S306 to D6S1571. YAC inser t sizes were determined by pulsed field gel electrophoresis (PFGE). Ch imerism of YACs was determined by fluorescent in situ hybridization (F ISH), and their integrity was determined by fingerprinting with Alu-PC R. We have identified 10 new CA repeat loci in this region as well as over 50 novel STSs, several tRNA genes, a new histone H2B gene and the phospholipase D gene. Using these new markers, we have rapidly genera ted a bacterial clone contig of over 250 kb, spanning the markers D6S1 260 to D6S1918 (WI-3111) with STSs spaced on average every 6 kb.