J. Membrillohernandez et Rk. Poole, BACTERIAL FLAVOHAEMOGLOBINS - A CONSENSUS SEQUENCE AND IDENTIFICATIONOF A DISCRETE ENTEROBACTERIAL GROUP AND OF FURTHER BACTERIAL GLOBINS, FEMS microbiology letters, 155(2), 1997, pp. 179-184
The amino acid sequences of haemoglobin-like proteins from the bacteri
a Alcaligenes eutrophus, Bacillus subtilis, Erwinia chrysanthemi, Esch
erichia coli, Vibrio parahaemolyticus, Vitreoscilla sp. and the yeast
Saccharomyces cerevisiae were studied. Phylogenies based on distance a
nd parsimony analysis showed that the eubacterial group can be easily
distinguished from the other haemoglobin-like proteins. The constructi
on of a consensus bacterial flavohaemoglobin based on the alignment of
six bacterial and one yeast globins allowed the design of consensus p
rimers to search for haemoglobin-like genes in other bacteria. PCR pro
ducts of the expected size were;found in Campylobacter jejuni, Salmone
lla typhimurium, Listeria monocytogenes, Rhizobium leguminosarum, Kleb
siella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus.