Citation
M. Roos et al., IMPROVED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PEPTIDOGLYCAN ISOLATED FROM VARIOUS STAPHYLOCOCCUS-AUREUS STRAINS FOR MASS-SPECTROMETRIC CHARACTERIZATION, Journal of chromatography B. Biomedical sciences and applications, 705(2), 1998, pp. 183-192
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273
→ ACNP
Volume
705
Issue
2
Year of publication
1998
Pages
183 - 192
Database
ISI
SICI code
0378-4347(1998)705:2<183:IHLSOP>2.0.ZU;2-5
Abstract
Reversed-phase high-performance liquid chromatography (RP-HPLC) of mur
opeptides, obtained by muramidase digestion of peptidoglycan in combin
ation with amino acid analysis and plasma desorption time-of-flight ma
ss spectrometry is today by far the best tool to analyze the fine stru
cture of the peptidoglycans. Here we report further improvements of th
e RP-HPLC separation of muropeptides for analyzing the peptidoglycans
of various methicillin-resistant strains of Staphylococcus aureus, wit
h emphasis on a more detailed characterization of the interpeptide bri
dge of the peptidoglycans of this species. (C) 1998 Elsevier Science B
.V.