SEPARATION OF PROTEOLYTIC-ENZYMES ORIGINATING FROM ANTARCTIC KRILL (EUPHAUSIA-SUPERBA) BY CAPILLARY ELECTROPHORESIS

Extracts prepared from Antarctic krill (Euphausia superba), mainly con sisting of acidic proteolytic enzymes, have been studied with capillar y electrophoretic techniques. Approximately 50 repeatable peaks were o btained with capillary zone electrophoresis on an untreated fused-sili ca capillary using a phosphate buffer containing anionic and cationic fluorosurfactant additives as separation medium. A faster separation w as achieved on a polyvinyl alcohol coated capillary. Quantitative vari ations of individual proteins regarding different krill enzyme batches were noted. In the krill samples trypsin-like serine proteinase, carb oxypeptidase A and carboxypeptidase B were tentatively identified. (C) 1998 Elsevier Science B.V.