APPLICATION OF CAPILLARY ELECTROPHORESIS TO THE SEPARATION OF STRUCTURALLY DIVERSE N-(SUBSTITUTED)-GLYCINE-PEPTOID COMBINATORIAL MIXTURES

The capillary electrophoresis (CE)-based separation of five N-(substit uted)-glycine (NSG)-peptoid mixtures with a wide range of physical and chemical properties was studied. A CE separation, initially developed using a single representative peptoid mixture, with a backround elect rolyte (BGE) modified by the addition of both methyl-beta-cyclodextrin and heptane sulfonic acid was found to provide good separations of mo st of the combinatorial mixtures investigated. For those mixtures not separated well by this procedure, the use of SDS micelles in conjuncti on with methyl-beta-cyclodextrin resulted in dramatic improvements in the separation. While no single set of separation conditions proved su fficient for all of the NSG-peptoid combinatorial mixtures, the two me thods were able to provide separation sufficient for characterization of a set of mixtures with a wide range of physical and chemical proper ties. The efficiency of the CE-based separation of the combinatorial m ixtures studied was compared to a reversed-phase liquid chromatographi c method using gradient elution. (C) 1998 Elsevier Science B.V.