AUTOREACTIVE B-CELL REGULATION - PERIPHERAL INDUCTION OF DEVELOPMENTAL ARREST BY LUPUS-ASSOCIATED AUTOANTIGENS

Citation
S. Santullimarotto et al., AUTOREACTIVE B-CELL REGULATION - PERIPHERAL INDUCTION OF DEVELOPMENTAL ARREST BY LUPUS-ASSOCIATED AUTOANTIGENS, Immunity, 8(2), 1998, pp. 209-219
Citations number
84
Categorie Soggetti
Immunology
Journal title
ISSN journal
10747613
Volume
8
Issue
2
Year of publication
1998
Pages
209 - 219
Database
ISI
SICI code
1074-7613(1998)8:2<209:ABR-PI>2.0.ZU;2-C
Abstract
Anti-Sm and anti-ssDNA transgenic (Tg) mice were generated using the V -H-D-J(H) rearrangement of an anti-Sm hybridoma of MRL/Mp-lpr/lpr orig in. B cells of each specificity account for 15%-35% of the splenic rep ertoire, but no circulating anti-Sm or anti-ssDNA antibodies are detec ted. Most autoreactive cells exhibit an immature B cell phenotype and have short half-lives equivalent to those of non-Tg immature B cells. However, at least some anti-Sm B cells are functional, because immuniz ation with murine snRNPs induces anti-Sm secretion. We propose that an ti-Sm and anti-ssDNA are eliminated during the transition to mature B cells and that this late stage of tolerance induction is consequential to their spontaneous activation in murine lupus.