MORPHOLOGY AND PROLIFERATION OF B16 MELANOMA-CELLS IN THE PRESENCE OFLANTHANOID AND AL3+ IONS

The effects of trivalent metal ions such as lanthanoid (La3+, Ce3+, Nd 3+, Sm3+, Gd3+, Er3+, Yb3+, Lu3+) and Al3+ ions on the morphological c hange and proliferation of B16 melanoma cells in culture are discussed . These metal ions induced morphological transformations and decreased growth rates at doses of 1 mM. B16 melanoma cells treated with La3+, Ce3+, Nd3+, Sm3+, and Gd3+ showed polyhedrical spreading. Elongation o f axones was dependent on the metal ions. B16 melanoma cells treated w ith Er3+, Yb3+, Lu3+, and Al3+ showed a long slender shape. Growth rat es of melanoma cells in the presence of 1 mM of metal ions (La3+, Ce3, Nd3+, Sm3+, Gd3+, Yb3+, Al3+) were significantly lower than that of control cells. Measurements of cell cycle indicated that the metal ion s arrested the transitions from G(0)/G(1) to S state.