SHARED DETERMINANTS OF RECEPTOR-BINDING FOR SUBTYPE-SELECTIVE, AND DUAL ENDOTHELIN-ANGIOTENSIN ANTAGONISTS ON THE AT(1) ANGIOTENSIN-II RECEPTOR

Site-directed interspecies amino acid exchange mas used to compare the binding determinants of a novel dual endothelial-angiotensin receptor Ligand, L-746,072, with type-1 angiotensin receptor (AT(1)) selective antagonists on AT receptors expressed in COS cells, These studies sug gest that residues on AT receptors which are non-conserved between amp hibian and mammalian species play a greater role in subtype selective ligand recognition than for dual receptor ligands, These data also sup port the hypothesis that a common non-peptide binding site exists with in transmembrane domains on peptidergic receptors. (C) 1998 Federation of European Biochemical Societies.