A. Szczepaniak et al., THE RIESKE PROTEIN FROM PURPLE SULFUR BACTERIA IS AN EXTRINSIC PROTEIN, Zeitschrift fur Naturforschung. C, A journal of biosciences, 53(1-2), 1998, pp. 15-20
The mode of membrane attachment of the Rieske iron-sulfur protein from
cytochrome bc(1) complex of Rhodospirillum rubrum has been studied us
ing biochemical approaches. In contrast to cytochrome c(1) the bacteri
al Rieske protein was extracted from chromatophores using chaotropic a
gents (NaSCN, urea: guanidine), an alkaline pH and relatively low conc
entration of Triton X-100. The results presented here lead to the conc
lusion, that the Rieske protein from chromatophores is extrinsic and t
hat their association with the rest of the complex involves hydrophobi
c interactions.