A NEW INHIBITOR OF SYNOVIAL PHOSPHOLIPASE A(2) FROM FERMENTATIONS OF PENICLLLIUM SP-62-92

Penidiamide, a new tripetide containing dehydrotryptamine, glycine and anthranilic acid linked together by two amide bonds, and oxindole wer e isolated from submerged cultures of Penicillium sp. 62-92. Both comp ounds preferentially inhibited human synovial phospholipase A(2), peni diamide with an IC50 Of 30 mu M and oxindole of 380 mu M. With the exc eption of U 937 cells (leukemia, human), no cytotoxic activities were detected against HL-60-(leukemia, human), HeLa S3-(epitheloid carcinom a, human), BHK 21-(kidney fibroblasts, hamster), and L1210-cells (leuk emia, mouse). No antimicrobial activity was detected for oxindole, and only weak antibacterial activity for penidiamide. The structure of pe nidiamide was elucidated by spectroscopic methods.