INTERACTION OF HISTONE H1 WITH CISPLATINUM MODIFIED DNA

Cis-diamminedichloroplatinum(II) (cis-DDP) is known as an effective an ticancer drug. Its therapeutic effect is supposed to be a consequence of the covalent binding to DNA. A number of cellular proteins were fou nd to bind selectively to DNA modified by cis-DDP (but not by its isom er trans-DDP). Here we present our observations on interaction of the linker histone H1 with cis-and trans-DDP modified DNA fragments. The r esults afford new experimental information about the preferential bind ing of histone H1 to cis-DDP-distorted DNAs versus trans-DDP modified ones.