CAPILLARY ZONE ELECTROPHORETIC SEPARATION OF BASIC-PROTEINS AND DRUGSUSING GUARAN AS A BUFFER MODIFIER

Guaran, a neutral polysaccharide, has been used as a buffer modifier t o improve the separation of basic proteins and drugs. Migration reprod ucibility, peak shape and efficiency were improved when 0.1 % guaran w as added to the buffer. The concentration of guaran, ionic strength, a nd pH of buffer solution were optimized to obtain the optimum separati on of proteins. Possible separation efficiencies of 700,000 plates per meter were obtained for test proteins. The relative standard deviatio n (%RSD) of the migration time of all test proteins was less than 0.5 %. Improved separation of beta-blockers was also observed when guaran was added to the buffer.