INVESTIGATION OF UNEXPECTED MIGRATION BEHAVIOR OF ADENOSINE IN CAPILLARY-ZONE-ELECTROPHORESIS

The capillary zone electrophoresis of two common nucleosides, adenosin e and inosine, was investigated. Both compounds were resolved in a 0.1 M sodium phosphate buffer, pH 7.5. Contrary to expectations, adenosin e behaved at this pH - 5 pH units lower than the literature pK(a) - as a negative ion, migrating behind mesityloxide (neutral marker) when w orking in normal polarity mode. To confirm the migration order, peaks were identified from absorption maxima, by high-speed scanning detecto r. The change in electrophoretic mobility with pH was investigated for the nucleosides, and 10 other background electrolytes were tried to m atch the separation capabilities of the sodium phosphate buffer. Most inorganic buffers showed comparable separation, while organic, Good-ty pe buffers lacked selectivity.