A. Lesnehulin et al., CLINICAL PHARMACOKINETICS OF AMIKACIN-TEI COPLANIN IN INFECTED SEVERELY BURNED PATIENTS - A PILOT-STUDY, Medecine et maladies infectieuses, 27(3), 1997, pp. 306-313
A pilot study of kinetics (concerning both serum and urine), clinical
tolerance, and microbiological efficacy of an amikacin (AMK) and teico
planin (TCN) combination was performed in 6 (35.3 +/- 13.2 years of ag
e, 68.3 +/- 8.6 kg) infected severely burned patients. Mean body surfa
ce area affected by 3rd degree burns was 43.0 +/- 23.8 %. Two daily 10
mg/kg (or 683 +/- 76 mg) doses of AMK were infused in 30 min., TCN wa
s infused in 10 min. at the loading dose of 12 mg/kg/12 h during 48 h,
then 12 mg/kg/24 h (or 820 +/- 90 mg) at steady state. Two series of
blood samples were drawn after the 1st (D1), and respectively the 9th
(AMK), and the 7th (TCN) (D5 at steady state) dose. Urine was collecte
d at D1 during the first part of the study. Serum and urinary AMK and
TCN levels were measured by FPIA and HPLC respectively. A non-compartm
ental method was used for kinetics and graphic analysis of both antibi
otics. Tolerance to and efficacy of treatment were good. There was no
systemic accumulation of antibiotic combination and their kinetics rem
ains linear at steady state. The mean duration of treatment was 8.1 +/
- 2.9 and 7.8 +/- 2.7 days respectively for AMK and TCN. There was no
statistically significant differences between parameters determined at
D1 and D5. Peak AMK concentrations (Cmax) were between 33.5 and 53.0
mg/l. Furthermore, for 5/6 subjects, 10 hours after the beginning of t
he treatment, serum concentrations were inferior to the assay's Limit
of quantification (< 0.6 mg/l). Peak TCN concentrations were relativel
y high (i.e. 91.9 to 197.5 mg/l), Cmin were somewhat inadequate (i.e.
Cmin 12 h (D1) = 9.0 +/- 4.4 mg/l and Cmin 24 h (D5) = 7.6 +/- 3.4 mg/
l. A new dosage was calculated on the basis of these experimental resu
lts. The aim was to obtain: 1) a Cmax value superior or equal to 50 mg
/l and a Cmin of the order of 0.9 mg/l for AMK; 2) a Cmax less than or
equal to to 100 mg/l and a Cmin greater than or equal to to 12 mg/l f
or TCN. Thus, the new regimen was set up as follows: 1) 12 mg/kg of AM
K every 12 h (i.e. 1,680 mg per day); 2) 10 mg/kg of TCN every 12 h (i
.e. 1,400 mg per day) and suppression of the loading dose. Furthermore
, both AMK and TCN were to be infused in 20 minutes. This new regimen
was submitted to the Ethics Committee and an assessment will be made o
n 10 more patients. The revision process described in this preliminary
study will optimize treatment for these patients.